ONKOSIGHT ADVANCED™ NEXT GENERATION SEQUENCING

GenPath^® proudly presents OnkoSight Advanced^TM, an exclusive product line offering both targeted and comprehensive next-generation sequencing assays designed for solid tumors and hematologic malignancies. Clinicians can opt for a focused panel, concentrating on actionable alterations in prevalent cancers, or a broader, more comprehensive panel. Our commitment is not only to deliver high-quality testing but also to address common challenges such as Turnaround Time (TAT) and Quantity Not Sufficient (QNS) rates. Backed by our in-house experts, OnkoSight Advanced empowers clinicians with precision and efficiency in cancer diagnostics.

OnkoSight Advanced Highlights:

Option for targeted or comprehensive panels
Industry-leading TATs and QNS rates
Tumor Mutation Burden (TMB) and Tumor-only Microsatellite Instability (MSI) included (on solid tumors, lymphoid, and multiple myeloma)
Whole genome Copy Number Variant (CNV) calling on select panels

Solid Tumor

General Solid Tumor Panel (50 genes, TMB, MSI, and Virtual Karyotyping*)

Test Code TJ16-5

AKT1	APC	ARID1A	ATM	BRAF	BRCA1	BRCA2	CDH1
CDKN2A	CTNNB1	EGFR	EPCAM	ERBB2	ERBB4	FBXW7	FGFR1
FGFR2	FGFR3	GNA11	GNAQ	GNAS	HRAS	IDH1	IDH2
KDR	KIT	KRAS	MEN1	MET	MLH1	MSH2	MSH6
NOTCH1	NRAS	PDGFRA	PIK3CA	PMS2	POLE	PTEN	PTPN11
RB1	RET	SMAD4	SMO	STK11	TERT	TSC1	TSC2
TP53	VHL

Bladder Cancer Panel (39 genes, TMB and MSI)

Test Code: TP51-9

ARID1A	ATM	BRAF	BRCA2	CCND1	CCNE1	CDKN2A	CREBBP
EGFR	EP300	ERBB2	ERBB3	ERCC2	FANCA	FBXW7	FGFR1
FGFR2	FGFR3	HRAS	KDM6A	KMT2A	KMT2C	KMT2D	KRAS
LRP1B	MAP2K1	MDM2	NF1	NRAS	PIK3CA	PRKDC	PTEN
RB1	SMARCA4	SPEN	STAG2	TERT	TP53	TSC1

Breast Cancer Panel (50 genes, TMB, MSI, and Virtual Karyotyping*)

Test Code: TP57-6

AKT1	AR	ATM	ATR	ATRX	BARD1	BRAF	BRCA1
BRCA2	BRIP1	CCNE1	CDH1	CDK12	CHEK1	CHEK2	EGFR
EPCAM	ERBB2	ERBB3	ERBB4	ESR1	FANCA	FANCC	FANCL
FGFR1	FGFR2	FOXA1	KRAS	MLH1	MRE11A	MSH2	MSH6
MUTYH	MYC	NBN	PALB2	PIK3CA	PMS2	PTEN	RAD50
RAD51	RAD51B	RAD51C	RAD51D	RAD54L	RB1	RECQL4	TERT
TP53	XRCC2

Colorectal Cancer Panel (32 genes, TMB, and MSI)

Test Code: TH50-8

AKT1	APC	ATR	BRAF	CHEK1	EGFR	EPCAM	ERBB2	ERBB3
ERBB4	FGFR1	FGFR2	FGFR3	FGFR4	HRAS	KRAS	MET	MAP2K1
MLH1	MSH2	MSH6	NRAS	NTRK1	PIK3CA	PMS2	POLD1	POLE
PTEN	SMAD4	STK11	TERT	TP53

Central Nervous System Tumor Panel (30 genes, TMB, and MSI, and Virtual Karyotyping*)

Test Code: TH57-3

APC	ATR	ATRX	BRAF	CDKN2A	CHEK1	CTNNB1	EGFR	ERBB2
H3F3A	H3F3B	H3F3C	HIST1H3B	HIST1H3C	IDH1	IDH2	MET	MYC
MYCN	NF1	NF2	NTRK1	PDGFRA	PIK3CA	PTEN	SMARCA4	SMARCB1
TERT	TP53	VHL

Gastrointestinal Stromal Tumor Panel (10 genes, TMB, and MSI)

Test Code: TH58-1

BRAF	ERBB3	KIT	NF1	NTRK1	PDGFRA	SDHA	SDHB	SDHC
SDHD

Gynecologic Neoplasms Panel (50 genes, TMB, MSI, and Virtual Karyotyping*)

Test Code: TH53-2

AKT1	AR	ATM	BRAF	BRCA1	BRCA2	BRIP1	CDH1	CDK12
CHEK1	CHEK2	EGFR	EPCAM	ERBB2	ERBB3	ESR1	FANCA	FANCC
FANCL	FGFR1	FGFR2	FOXA1	KRAS	MLH1	MRE11A	MSH2	MSH6
MUTYH	NBN	MYC	NF1	NTRK1	PALB2	PIK3CA	PMS2	POLE
PTEN	RAD50	RAD51B	RAD51C	RAD51D	RAD54L	RB1	RECQL4	SLX4
SMARCA4	TBX3	TERT	TP53	XRCC2

Melanoma Panel (27 genes, TMB, and MSI)

Test Code: TH59-9

BAP1	BRAF	BRCA2	CDK4	CDKN2A	CTNNB1	EIF1AX	GNA11	GNAQ
GNAS	KIT	MAP2K1	MITF	NBN	NF1	NRAS	NTRK1	PALB2
PDGFRA	PIK3CA	PTEN	RB1	SF3B1	SMO	SRC	TERT	TP53

Lung Cancer Panel (23 genes, TMB, and MSI)

Test Code: TH49-0

AKT1	ALK	ATR	BRAF	CHEK1	DDR2	EGFR	ERBB2	ERBB3
FGFR1	HRAS	KRAS	MAP2K1	MET	NRAS	NTRK1	PIK3CA	POLD1
POLE	ROS1	STK11	TERT	TP53

Pancreaticobiliary Tumor Panel (23 genes, TMB, MSI, and Virtual Karyotyping*)

Test Code: TK84-0

ARID1A	BRAF	BRCA1	BRCA2	CCNE1	ERBB2	FGFR1	FGFR2	FGFR3
IDH1	IDH2	KRAS	MLH1	MSH2	MSH6	NRAS	PALB2	PIK3C2G
PMS2	STK11	TGFBR2	TP53	VHL

Prostate Cancer Panel (49 genes, TMB, MSI, and Virtual Karyotyping*)

Test Code: TH48-2

AKT1	AR	ARID1A	ATM	ATR	BARD1	BRCA1	BRCA2
BRAF	BRIP1	CCND1	CCNE1	CDK12	CHD1	CHEK1	CHEK2
CTNNB1	EPCAM	FAM175A	FANCA	FANCD2	FANCL	FOXA1	FOXO1
GEN1	HOXB13	HRAS	IDH1	MLH1	MRE11A	MSH2	MSH6
MUTYH	MYC	NBN	NTRK1	PALB2	PIK3CA	PMS2	PPP2R2A
PTEN	RAD51B	RAD51C	RAD51D	RAD54L	RB1	SPOP	TERT
TP53

Chronic Lymphoid Neoplasm

Chronic Lymphoid Neoplasm Panel (41 genes, TMB, MSI, and Virtual Karyotyping*)

Test Code: TH55-7 (for BM and PB specimen); TJ93-4 (for FFPE specimen)

ATM	BCL2	BCL6	BIRC3	BRAF	BTK	CARD11	CD79B	CDKN2A
CREBBP	CXCR4	DNMT3A	EP300	EZH2	IDH1	IDH2	IKZF1	IRF4
JAK1	JAK3	KMT2D	KRAS	MAP2K1	MEF2B	MYC	MYD88	NOTCH1
NOTCH2	NRAS	PIK3CA	PIK3CG	PLCG2	PTPRD	RHOA	SETD2	SF3B1
STAT3	STAT5B	TET2	TNFRSF14	TP53

Multiple Myeloma

Multiple Myeloma Panel (50 genes, TMB, MSI, and Virtual Karyotyping*)

Test Code: TL67-3 (for BM and PB specimen); TL68-1 (for FFPE specimen)

AKT1	AKT2	AKT3	ATM	ATR	B2M	BIRC3	BRAF	CCND1
CDK4	CDKN1B	CDKN2A	CXCR4	CYLD	DIS3	DNMT3A	EGFR	FAM46C
FGFR3	FUBP1	IDH1	IDH2	IGF1R	IKZF1	IRF4	JAK2	KDM6A
KRAS	MAX	MYC	MYD88	NF1	NFKBIA	NOTCH1	NRAS	PIK3CA
PIK3CG	PIK3R1	PIK3R2	PIM1	PRDM1	PSMB8	PTPN11	RB1	STAT3
TERT	TET2	TGFBR2	TP53	TRAF2

Myeloid Malignancies

Myeloid Complete Panel (148 genes)

Test Code: M374-7

75-gene DNA (Test code TM00-2)

73-gene Fusions (Test code TP02-2)

Comprehensive Myeloid Panel (75 genes)

Test Code: TM00-2

ABL1	ANKRD26	ASXL1	ATRX	BCOR	BCORL1	BRAF	BTK	CALR
CBL	CBLB	CBLC	CCND2	CDC25C	CDKN2A	CEBPA	CSF3R	CUX1
CXCR4	DCK	DDX41	DHX15	DNMT3A	ETNK1	ETV6	EZH2	FBXW7
FLT3	GATA1	GATA2	GNAS	HRAS	IDH1	IDH2	IKZF1	JAK2
JAK3	KDM6A	KIT	KMT2A	KRAS	LUC7L2	MAP2K1	MPL	MYC
MYDD88	NF1	NOTCH1	NPM1	NRAS	PDGFRA	PHF6	PPM1D	PTEN
PTPN11	RAD21	RBBP6	RPS14	RUNX1	SETBP1	SF3B1	SH2B3	SLC29A1
SMC1A	SMC3	SRSF2	STAG2	STAT3	TWT2	TP53	U2AF1	U2AF2
WT1	XPO1	ZRSR2

General Myeloid Panel (50 genes)

Test Code: TL95-4

ABL1	ANKRD26	ASXL1	ATRX	BCOR	BCORL1	BRAF	CALR	CBL
CCND2	CDKN2A	CEBPA	CSF3R	CUX1	DDX41	DNMT3A	ETNK1	ETV6
EZH2	FBXW7	FLT3	GATA2	HRAS	IDH1	IDH2	JAK2	KDM6A
KIT	KMT2A	KRAS	MAP2K1	MPL	MYD88	NF1	NPM1	NRAS
PDGFRA	PHF6	PTEN	PTPN11	RUNX1	SETBP1	SF3B1	SRSF2	STAG2
TET2	TP53	U2AF1	WT1	ZRSR2

Acute Myeloid Leukemia (AML) Panel (30 genes)

Test Code: TL94-7

ASXL1	BRAF	BCOR	CEBPA	CSFR3	DDX41	DNMT3A	ETV6	EZH2
FLT3	GATA2	HRAS	IDH1	IDH2	JAK2	KIT	KMT2A	KRAS
NF1	NPM1	NRAS	PDGFRA	PHF6	PTPN11	RUNX1	SETBP1	STAG2
TET2	TP53	WT1

Myeloproliferative Neoplasms (MPN) Panel (17 genes)

Test Code: TL96-2

ABL1	ASXL1	CALR	CBL	CSF3R	DNMT3A	EZH2	IDH1	IDH2
JAK2	KIT	MPL	SETBP1	SF3B1	SRSF2	TP53	U2AF1

MPN Cascade Sequencing Panel (3 genes)

Test Code: TL97-4

JAK2 (exon 12 and V617F)

CALR

MPL

Myelodysplastic Syndrome (MDS) Panel (18 genes)

Test Code: TL98-8

ASXL1	BCOR	CBL	DNMT3A	ETV6	EZH2	JAK2	KRAS	NRAS
PTPN11	RUNX1	SETBP1	SF3B1	SRSF2	TET2	TP53	U2AF1	ZRSR2

*Virtual karyotyping or CNA assessment will require samples with >50% tumor content. Normal findings will not be reported. For TJ16, CNA assessment will be performed on samples with an indication for tumor subtypes with HRD-associated therapy including high-grade serous ovarian (HGSO), fallopian tube and mesenteric, prostate, breast, and pancreaticobiliary.

GenPath is one of the select National Cancer Institute- Molecular Analysis for Therapy Choice (NCI-MATCH) clinical trials network laboratories, with DLAP approved solid tumor assays.

Healthcare providers should only order panels if each gene or test in the panel is medically necessary.

ONKOSIGHT ADVANCED™ NEXT GENERATION SEQUENCING

Solid Tumor

Test Code TJ16-5

AKT1

APC

ARID1A

ATM

BRAF

BRCA1

BRCA2

CDH1

CDKN2A

CTNNB1

EGFR

EPCAM

ERBB2

ERBB4

FBXW7

FGFR1

FGFR2

FGFR3

GNA11

GNAQ

GNAS

HRAS

IDH1

IDH2

KDR

KIT

KRAS

MEN1

MET

MLH1

MSH2

MSH6

NOTCH1

NRAS

PDGFRA

PIK3CA

PMS2

POLE

PTEN

PTPN11

RB1

RET

SMAD4

SMO

STK11

TERT

TSC1

TSC2

TP53

VHL

Test Code: TP51-9

ARID1A

ATM

BRAF

BRCA2

CCND1

CCNE1

CDKN2A

CREBBP

EGFR

EP300

ERBB2

ERBB3

ERCC2

FANCA

FBXW7

FGFR1

FGFR2

FGFR3

HRAS

KDM6A

KMT2A

KMT2C

KMT2D

KRAS

LRP1B

MAP2K1